rabbit anti-human st2 antibody  (Millipore)

Journal: The Journal of investigative dermatology

Article Title: Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.

doi: 10.1016/j.jid.2018.10.048

Figure Lengend Snippet: Figure 1. Skin IL-33/ST2 expression and ILC2s numbers between healthy control subjects and patients with DRESS. (a) Representative histopathological features of skin biopsies from healthy control subjects and DRESS patients are shown. (H&E stain; original magnification 200). High ST2 (b) and IL-33 (c) expression in the dermis skin and (d) TSLP expression in the epidermis skin from DRESS patients (n ¼ 5) and controls (n ¼ 5) by immunohistochemical staining.

Article Snippet: Rabbit monoclonal anti-human TSLP antibody (GTX85059; Genetex, Irvine, CA), rabbit monoclonal anti-human IL-33 antibody (12372- 1-AP; Proteintech Rosemont, IL), and rabbit monoclonal anti- human ST2 antibody (11920-1-AP; Proteintech) were diluted 1:500 with antibody diluent and applied to the tissues for 30 mi- nutes at room temperature.

Techniques: Expressing, Control, Staining, Immunohistochemical staining

Journal: The Journal of investigative dermatology

Article Title: Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.

doi: 10.1016/j.jid.2018.10.048

Figure Lengend Snippet: Figure 2. Dynamic ILC2s populations and cytokine expression in patients with DRESS. (a) The percentage of blood ILC2s with lymphoid gate was higher in the active stage in DRESS patients (n ¼ 15) than control subjects (n ¼ 15), and the number of blood ILC2s were decreased after steroid therapy at 4 weeks resolution phase. Furthermore, serum IL-5 (b), eotaxin (c), IL-33 (d), soluble ST2 (e), and TLSP (f) levels in DRESS patients from baseline to the 4-week follow-up are shown. DRESS, drug reaction with eosinophilia and systemic symptoms; ILC2, type 2 innate lymphoid cell; NS, not significant.

Article Snippet: Rabbit monoclonal anti-human TSLP antibody (GTX85059; Genetex, Irvine, CA), rabbit monoclonal anti-human IL-33 antibody (12372- 1-AP; Proteintech Rosemont, IL), and rabbit monoclonal anti- human ST2 antibody (11920-1-AP; Proteintech) were diluted 1:500 with antibody diluent and applied to the tissues for 30 mi- nutes at room temperature.

Techniques: Expressing, Control

Journal: The Journal of investigative dermatology

Article Title: Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.

doi: 10.1016/j.jid.2018.10.048

Figure Lengend Snippet: Figure 3. The associations of soluble ST2. The graphs of the relationship between serum soluble ST2 versus IL-33, ALT, eosinophils, IL-5, eotaxin, and TSLP at the onset of patients with DRESS. ALT, alanine aminotransferase; Rs, Spearman’s r correlation coefficient.

Article Snippet: Rabbit monoclonal anti-human TSLP antibody (GTX85059; Genetex, Irvine, CA), rabbit monoclonal anti-human IL-33 antibody (12372- 1-AP; Proteintech Rosemont, IL), and rabbit monoclonal anti- human ST2 antibody (11920-1-AP; Proteintech) were diluted 1:500 with antibody diluent and applied to the tissues for 30 mi- nutes at room temperature.

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

doi: 10.12659/MSM.921863

Figure Lengend Snippet: The primer sequences for Real-time qPCR.

Article Snippet: The primary antibodies were rabbit anti-human IL-33 polyclonal antibody (Abcam, UK), and rabbit anti-human ST2 polyclonal antibody (Proteintech, USA).

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

doi: 10.12659/MSM.921863

Figure Lengend Snippet: The real-time qPCR results show that IL-33 and its receptor ST2 are highly expressed in AB tissues compared to NOM tissues at the mRNA level. ( A ) IL-33; ( B ) ST2. * p<0.05; **** p<0.0001.

Article Snippet: The primary antibodies were rabbit anti-human IL-33 polyclonal antibody (Abcam, UK), and rabbit anti-human ST2 polyclonal antibody (Proteintech, USA).

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

doi: 10.12659/MSM.921863

Figure Lengend Snippet: Western blot analysis results show that IL-33 and its receptor ST2 are highly expressed in AB tissues compared to NOM tissues at the protein level. ( A ) IL-33; ( B ) ST2. ***p<0.001.

Article Snippet: The primary antibodies were rabbit anti-human IL-33 polyclonal antibody (Abcam, UK), and rabbit anti-human ST2 polyclonal antibody (Proteintech, USA).

Techniques: Western Blot

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

doi: 10.12659/MSM.921863

Figure Lengend Snippet: Immunohistochemistry results show the expression of ( A ) IL-33 and ( B ) ST2 in NOM.

Article Snippet: The primary antibodies were rabbit anti-human IL-33 polyclonal antibody (Abcam, UK), and rabbit anti-human ST2 polyclonal antibody (Proteintech, USA).

Techniques: Immunohistochemistry, Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

doi: 10.12659/MSM.921863

Figure Lengend Snippet: Immunohistochemistry results show the expression of ST2 in AB. ( A ) Solid/multicystic AB (200×); ( B ) peripheral AB (200×); ( C ) desmoplastic AB (200×); ( D ) unicystic AB (200×); ( E ) monocyte (400×); ( F ) plasmocyte and lymphocyte (400×); ( G ) osteoclast (400×); ( H ) negative control (200×).

Article Snippet: The primary antibodies were rabbit anti-human IL-33 polyclonal antibody (Abcam, UK), and rabbit anti-human ST2 polyclonal antibody (Proteintech, USA).

Techniques: Immunohistochemistry, Expressing, Negative Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

doi: 10.12659/MSM.921863

Figure Lengend Snippet: The difference of IL-33 and ST2 expression between AB and NOM tissues.

Article Snippet: The primary antibodies were rabbit anti-human IL-33 polyclonal antibody (Abcam, UK), and rabbit anti-human ST2 polyclonal antibody (Proteintech, USA).

Techniques: Expressing

Journal: The Journal of allergy and clinical immunology

Article Title: The role of ST2 and ST2 genetic variants in schistosomiasis

doi: 10.1016/j.jaci.2016.12.969

Figure Lengend Snippet: Association between the 6 ST2 SNPs typed in this study and sST2 levels Linear regression was used to test the association between a SNP and log10 transformed adjusted sST2 (adjusted for sex, age, HBV infection, alcohol consumption and plate factors), and stage of disease was included as a covariate in the model.

Article Snippet: Sections were treated with 5% BSA at room temperature for 20 minutes before being incubated with rabbit anti-human ST2 antibody (#A1913, ABclonal, MA, USA, diluted in 1:200) at room temperature for one hour.

Techniques: Transformation Assay, Infection

Journal: The Journal of allergy and clinical immunology

Article Title: The role of ST2 and ST2 genetic variants in schistosomiasis

doi: 10.1016/j.jaci.2016.12.969

Figure Lengend Snippet: (A) Representative images of normal liver tissues, HBV-induced liver fibrosis tissues and Schistosome japonicum-induced liver fibrosis tissues (Images were captured at 100X with scale bar of 200 μm). (B) Comparison of scores of ST2 staining intensity between the three groups.

Article Snippet: Sections were treated with 5% BSA at room temperature for 20 minutes before being incubated with rabbit anti-human ST2 antibody (#A1913, ABclonal, MA, USA, diluted in 1:200) at room temperature for one hour.

Techniques: Comparison, Staining

Journal: Molecular Medicine Reports

Article Title: IL-33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin α4β1 and CD62L

doi: 10.3892/mmr.2017.7085

Figure Lengend Snippet: IL-33/ST2 is co-expressed in trophoblasts. Immunohistochemical analysis (n=10) for IL-33 and ST2 expression in villi tissues from healthy women at the first-trimester of a normal pregnancy. Magnification, ×200 or ×400. IL-33, interleukin 33; ST2, IL-1 receptor-like 1.

Article Snippet: Thereafter, the samples were incubated with rabbit anti-human IL-33 antibody (1 µg/ml; cat. no. HPA02442; Sigma-Aldrich; Merck Millipore, Darmstadt, Germany), rabbit anti-human ST2 antibody (10 µg/ml; cat. no. PRS3363; Sigma-Aldrich; Merck Millipore) or rabbit IgG isotype (1:2,000; cat. no. GTX35035; GeneTex, Inc., Irvine, CA, USA) overnight at 4°C in a humidified chamber.

Techniques: Immunohistochemical staining, Expressing

Journal: Molecular Medicine Reports

Article Title: IL-33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin α4β1 and CD62L

doi: 10.3892/mmr.2017.7085

Figure Lengend Snippet: Trophoblast cell lines express ST2. Expression of ST2 in JEG3, JAR, BeWo and HTR8 cells was determined by flow cytometry. The data are expressed as the mean ± standard error of the mean. **P<0.01 and ***P<0.01 vs. HTR8 group. ST2, interleukin 1 receptor-like 1; NS, no statistical significance.

Article Snippet: Thereafter, the samples were incubated with rabbit anti-human IL-33 antibody (1 µg/ml; cat. no. HPA02442; Sigma-Aldrich; Merck Millipore, Darmstadt, Germany), rabbit anti-human ST2 antibody (10 µg/ml; cat. no. PRS3363; Sigma-Aldrich; Merck Millipore) or rabbit IgG isotype (1:2,000; cat. no. GTX35035; GeneTex, Inc., Irvine, CA, USA) overnight at 4°C in a humidified chamber.

Techniques: Expressing, Flow Cytometry

Journal: The Journal of allergy and clinical immunology

Article Title: The role of ST2 and ST2 genetic variants in schistosomiasis

doi: 10.1016/j.jaci.2016.12.969

Figure Lengend Snippet: Association between the 6 ST2 SNPs typed in this study and sST2 levels Linear regression was used to test the association between a SNP and log10 transformed adjusted sST2 (adjusted for sex, age, HBV infection, alcohol consumption and plate factors), and stage of disease was included as a covariate in the model.

Article Snippet: Sections were treated with 5% BSA at room temperature for 20 minutes before being incubated with rabbit anti-human ST2 antibody (#A1913, ABclonal, MA, USA, diluted in 1:200) at room temperature for one hour.

Techniques: Transformation Assay, Infection

Journal: The Journal of allergy and clinical immunology

Article Title: The role of ST2 and ST2 genetic variants in schistosomiasis

doi: 10.1016/j.jaci.2016.12.969

Figure Lengend Snippet: (A) Representative images of normal liver tissues, HBV-induced liver fibrosis tissues and Schistosome japonicum-induced liver fibrosis tissues (Images were captured at 100X with scale bar of 200 μm). (B) Comparison of scores of ST2 staining intensity between the three groups.

Article Snippet: Sections were treated with 5% BSA at room temperature for 20 minutes before being incubated with rabbit anti-human ST2 antibody (#A1913, ABclonal, MA, USA, diluted in 1:200) at room temperature for one hour.

Techniques: Staining